The Active Peptide from Shark Liver (APSL) was expressed in E.coli BL21 cells.The cDNA encoding APSL protein was obtained from shark regenerated hepatic tissue by RT-PCR, then it was cloned in the pET-28a expression vector.The expressed fusion protein was purified by Ni-IDA affinity chromatography.SDS-PAGE and HPLC analysis showed the purity of the
In this study, the precise orbit determination (POD) software is developed for optical observation.To improve the performance of Spot Treatment the estimation algorithm, a nonlinear batch filter, based on the unscented transform (UT) that overcomes the disadvantages of the least-squares (LS) batch filter, is utilized.The LS and UT batch filter algo
Istrian shorthaired hound is an old indigenous Croatian dog breed with historical traces of its origin, which date back to the 14th century.Due to its intelligence and great hunting abilities, it is considered an excellent hunting dog.Despite its ancient origin, there is no data on genetic diversity, bishop rotary population structure, and degree o